Detection of Pig DNA Contamination in Facial Creams Containing Collagen Using Conventional PCR

Authors

  • Meutia Srikandi Fitria Diploma Study Program of Medical Laboratory Technology, Universitas Muhammadiyah Semarang
  • Juliana Permata Dewi Diploma Study Program of Medical Laboratory Technology, Universitas Muhammadiyah Semarang
  • Ana Hidayati Mukaromah Magister of Clinical Laboratory Science, Postgraduate Program Universitas Muhammadiyah Semarang

Keywords:

cosmetics, collagen cream, cytochrome b, PCR

Abstract

Background: Cosmetics are pharmaceutical preparations intended to support appearance or care for the body. Both local and imported facial whitening products are mostly marketed at varying prices, from cheap to expensive, making more people buy them. The supply of collagen extracted from mammals has recently been limited and is mostly obtained from the tendons and skin of cattle and pigs. The collagen content not listed on cosmetic packaging makes people worried about pork collagen being contaminated, therefore detection is needed to find out. This research aimed to detect whether there was pork contamination in facial cream products containing collagen with Cytochrome b (Cyt-b) primers using conventional PCR. Method: The research design is exploratory. The samples used were 5 facial cream samples and one positive control. Samples were subjected to DNA extraction, DNA concentration quantification, PCR, and DNA electrophoresis. Result: In the positive control, a DNA band of ~149 bp specifically targeting Cytochrome b was formed, while the facial cream sample did not form a DNA band. Conclusion: The five facial creams used as samples were not contaminated with pork contamination.

Author Biography

Meutia Srikandi Fitria, Diploma Study Program of Medical Laboratory Technology, Universitas Muhammadiyah Semarang

First author, Corresponding author

References

Getachew, M., and Tewelde, T. Cosmetic Use and Its Adverse Events among Female Employees of Jimma University, Southwest Ethiopia. Ethiopian Journal Health Sciences. 2018. 28(6): 717-724. DOI: https://doi.org/10.4314%2Fejhs.v28i6.6

Fitrianingsih, S., Nafi’ah, L.N., and Ismah, K. Formulasi Krim dari Bahan Alam Pada Aktivitas Antiaging. Cendekia Journal of Pharmacy. 2022. 6(2): 318-325. DOI: https://doi.org/10.31596/cjp.v6i2.216.

Campos, L.D., Junior, V.A.S, Pimentel, J.D., Carrega, G.L.F., and Carazin, C.B.B. Collagen Supplementation in Skin and Orthopedic Diseases: A Review of The Literature. Heliyon. 2018. 9(4): 1-10. DOI: https://doi.org/10.1016/j.heliyon.2023.e14961.

Richard-Blum, S. The Collagen Family. Cold Spring Harbor Perspectives in Biology. 2011. 3(1): 1-9. DOI: https://doi.org/10.1101%2Fcshperspect.a004978

Rusmin. Formulasi dan Uji Stabilitas Sediaan Krim Ekstrak Rimpang Iris (Iris pallida Lamk.) Menggunakan Emulgator Anionik dan Nonionik. Jurnal Kesehatan. 2021. 5(2): 50-58. LINK: https://jurnal.yamasi.ac.id/index.php/Jurkes/article/view/167

Kim, Y. S., Yu, H. K., Lee, B.Z., Hong, K.W. Effect of DNA Extraction Methods on The Detection of Porcine Ingredients in Halal Cosmetics Using Real-Time PCR. Applied Biological Chemistry. 2018. 61(5): 549-555. DOI: https://doi.org/10.1007/s13765-018-0389-x.

Setyawati, R. and Zubaidah, S. Optimasi Konsentrasi Primer dan Suhu Annealing dalam Mendeteksi Gen Leptin pada Sapi Peranakan Ongole (PO) Menggunakan Polymerase Chain Reaction (PCR). Indonesian Journal of Laboratory. 2021. 4(1): 36-40. DOI: https://doi.org/10.22146/ijl.v4i1.65550.

Muflihah, Hardianto, A., Kusumaningtyas, P., Prabowo, S., and Hartati, Y.W. DNA-based Detection of Pork Content in Food. Heliyon. 2023. 9(3): 1-15. DOI: https://doi.org/10.1016/j.heliyon.2023.e14418.

Ishak, N. and Mutalib, S.A. Extraction and Detection of Animal Deoxyribonucleic Acid (DNA) Species on Lipstick using Polymerase Chain Reaction (PCR) Assay. International Journal of ChemTech Research. 2018. 11(06): 250-254. DOI: https://doi.org/10.20902/ijctr.2018.110632.

Astuti, R. Y. Deteksi Unsur Babi Pada Gelatin Menggunakan Teknologi Polymerase Chain Reaction (PCR) dengan Primer Spesifik dan Restriction Fragment Length Polymorphism (RFLP). Skripsi. 2017. Universitas Gadjah Mada Yogyakarta.

Adzakiyyi, M.L., Susilowati, T., Rokhim, S., and Rachmawati, Y. Sus sp. DNA Encoding Cyt b Gene Detection Test on Meat Grinding Samples Using Conventional PCR. Indonesian Journal of Halal Research. 2020. 2(2): 40-45. DOI: https://doi.org/10.15575/ijhar.v2i2.8489

Almaida, G.R., Oktanella, Y., and Ciptadi, G. Variasi Genetik Kambbing Senduro dan Peranakan Etawa (Pe) Berdasarkan Sekuen Gen Cyt-B (Cytochrome B) dengan Metode Polymerase Chain Reaction. Ternak Tropika Journal of Tropical Animal Production. 2020. 21(2): 102-110. DOI: https://doi.org/10.21776/ub.jtapro.2020.021.02.3.

Fatchiyah, Arumingtyas, E.L., Widyarti, S., dan Rahayu, S. 2016. Biologi Molekular: Prinsip Dasar Analisis. Jakarta: Penerbit Erlangga.

Nugroho, K., Widyajayantie, D., Ishthifaiyyah, S.A., and Apriliani, E. Pemanfaatan Teknologi Droplet Digital PCR (ddPCR) dalam Kegiatan Analisis Molekuler Tanaman. Jurnal Bios Logos. 2021. 11(1): 28-40. DOI: https://doi.org/10.35799/jbl.11.1.2021.31101

Mustafa, H., Widayati, A.N., Gunawan, and Rachmawaty, I. Variasi Genetik (Polimorfisme) Anopheles barbirostris dengan Menggunakan Metode PCR-RAPD. Prosiding Seminar Nasional Biologi FMIPA UNM. 8 Agustus 2020. Universitas Negeri Makassar.

Gupta, N. DNA Extraction and Polymerase Chain Reaction. Journal of Cytology. 2019. 36(2): 116-117. DOI: https://doi.org/10.4103/JOC.JOC_110_18

Dewanata, P.A. and Mushlih, M. Differences in DNA Purity Test Using UV-Vis Spectrophotometer and Nanodrop Spectrophotometer in Type 2 Diabetes Mellitus Patients. Indonesian Journal of Innovation Studies. 2021. 15: 6-10. DOI: https://doi.org/10.21070/ijins.v15i.553.

Purnamasari, Y. 2013. Varian Genetik Daerah Hipervariabel I (HVI) DNA Mitokondria Pada Empat Generasi dengan Riwayat Diabetes Melitus Tipe 2. Thesis. Universitas Pendidikan Indonesia. LINK: http://repository.upi.edu/3976/

Ludyasari, A., Susilowati, R., and Abidin, H.M. 2014. Pengaruh Suhu Annealing Pada Program PCR Terhadap Keberhasilan Amplifikasi DNA Udang Jari (Metapenaeus elegans De Man, 1907) Laguna Segara Anakan, Cilacap, Jawa Tengah. Thesis. Universitas Islam Negeri Maulana Malik Ibrahim.

Ischak, N.I. Isolasi DNA dan Protein dengan Tehnik PCR Elektroforesis Agarose dan SDS-PAGE. Jurnal Entropi. 2014. 9(1): 751-757. LINK: https://repository.ung.ac.id/karyailmiah/show/10535/netty-ino-ischak-isolasi-dna-dan-protein-dengan-teknik-pcr-elektroforesis-agarose-dan-sds-page.html#

Tilawah, S., Sari, R., anad Apridamayanti, P. Optimasi Volume DNA Marker dan Volume Amplifikasi Gen tetL Resistensi Antibiotik Tetrasiklin dari Bakteri Bacillus cereus pada Pasien Ulkus Diabetik. Jurnal Farmasi. 2019. 4(1): 1-7. LINK: https://jurnal.untan.ac.id/index.php/jmfarmasi/article/view/39968

Downloads

Published

2024-10-30

How to Cite

Fitria, M. S., Dewi, J. P., & Mukaromah, A. H. (2024). Detection of Pig DNA Contamination in Facial Creams Containing Collagen Using Conventional PCR. Prosiding Seminar Kesehatan Masyarakat [Proceeding of Public Health Seminar], (October), 1–9. Retrieved from https://jurnalnew.unimus.ac.id/index.php/prosidingfkm/article/view/628